a, Experimental design of SDR in mice fed 1 day on 3 days and 4 days on 6 days. AL, liberty. SDR (33.3% × 3), 33.3% food provided daily from day 1 to day 3. SDR (66.7% × 6), 66.7% food provided daily from day 1 to day 6. b–e1-d food for 3 days followed by re-feeding (< b>b–d) or 6 days (e) markedly increased body fat mass after a 4-day diet,n = 9 biologically independent mice for AL, SDR (10–25–65%) and SDR (66.7% × 6), SDR (65–25–10%) n = 7, and n 8 for SDR (33.3% * 3). f, representative images of iWAT isolated from the SDR(10–25–65%) group. Each square measures 0.5 cm by 0.5 cm. D4D, day 4 of the dark period; g,f The weight of iWAT at (n = 12 biologically independent mice per group). h,f Representative images of H&E-stained sections of iWAT in . Scale bar, 50 μm. i–k3d (i,j) or re-feeding after 2-dimensional feeding with 1-d diet for 2 days () significantly increased body fat mass. n = 8 biologically independent mice in AL and n = 9 animals. l,m12 days (l) or 24 days (m) Re-feeding after daily feeding of 66.7% food >) Body fat mass increased significantly (n = 9 biologically independent mice per group). nrefeeding after 15 cycles of alternate-day fasting significantly increased body fat mass (n = 9 biologically independent mice per group). b–e,i–k,or l–< に表示されるデータb>n was performed concurrently with a single control experiment. Data are presented as mean ± sd.Statistical significance is two-sided Student’s t determined by testing. aP. < 0.05; b,P. < 0.01; c, ***P. < 0.001; versus AL or D0 groups.Accurate P. Values are given in Source Data Figure 1. credit: Nature Metabolism (2022). DOI: 10.1038/s42255-022-00687-6″ width=”800″ height=”530″/> Re-feeding after various types of dietary restriction induces rapid accumulation of fat mass. a, Experimental design of SDR in mice fed 1 day on 3 days, or 4 days on 6 days. AL, liberty. SDR (33.3% × 3), 33.3% food provided daily from day 1 to day 3. SDR (66.7% × 6), 66.7% food provided daily from day 1 to day 6. b–ere-feeding after 1-d feeding in 3-d (b–d) or 4-d food in 6 d (e) markedly increased body fat mass, n= 9 biologically independent mice with AL, SDR (10–25–65%) and SDR (66.7% × 6), n = 7 (65–25–10%) for SDR, and n = 8 (33.3% × 3) for SDR. What, representative images of iWAT isolated from the SDR(10–25–65%) group. Each square measures 0.5 cm by 0.5 cm. D4D, day 4 of the dark period; giWAT weights What (n = 12 biologically independent mice per group). timerepresentative images of H&E-stained sections of iWAT WhatScale bar, 50 μm. Me–kre-feeding after 2-d feeding in 3-d (Me,j) or 2d (k) significant increase in body fat mass; n= 8 biologically independent mice with AL, n = 9 for other groups. l,metersrefeeding after 12 days of 66.7% food daily (l) or 24 days (meters) Significantly increased body fat mass (n = 9 biologically independent mice per group). nrefeeding after 15 cycles of alternate-day fasting significantly increased body fat mass (n= 9 biologically independent mice per group).data shown in b–e, Me–k Also l–n A single control experiment was run concurrently. Data are presented as mean ± sd.Statistical significance is for two-sided Student t-test. , P.< 0.05; b, P.< 0.01; c, ***P.< 0.001; versus AL or D0 groups.just P.Values are given in Figure 1 of the source data. credit: natural metabolism(2022). DOI: 10.1038/s42255-022-00687-6
Research published in natural metabolismA research group led by Professor Zhai Qiwei of the Shanghai Institute of Nutrition and Health, Chinese Academy of Sciences, reveals that post-diet increases in lactic acid bacteria and their metabolites promote intestinal lipid absorption, leading to rapid fat accumulation and obesity in mice. Did.
Also, this effect high protein diet Or certain antibiotics that inhibit the growth of lactic acid bacteria in the gut. An article commenting on this work was published in the News and Views section. natural metabolism.
Dietary restriction (DR), often referred to as dieting, is widely used to reduce fat mass and lose weight through restriction. food intakeHowever, weight gain after dieting is still a big problem.
In this study, researchers used 10 diet protocols to investigate the effect of post-diet ‘refeeding’ on fat mass in mice.All data show that post-diet refeeding leads to rapid fat mass accumulation and obesity. was white adipose tissue (WAT) and total lipid oxidation are the main causes of increased fat mass after dieting.
To identify potential dietary interventions to prevent increased fat mass after dieting, researchers fed mice either a high-protein diet, a low-protein diet, or a normal protein diet. Essential amino acids Post-diet stage.
Results showed that a high-protein diet prevented rapid fat mass accumulation and obesity, and even partially maintained diet-induced fat loss. Including pair-feeding experiments showed that diet composition was more important. calorie intake Blocks fat mass gain after dieting.
Further studies have shown that a post-diet, high-protein diet reduces intestinal and serum bile acid levels, attenuates intestinal lipid absorption, decreases the anabolic effect of WAT, and may also increase total lipid oxidation. clarified.
The researchers then analyzed the composition of the gut microbiota and found that refeeding a normal protein diet after a diet dramatically increased the amount of lactobacilli by approximately 50%, and refeeding a high protein diet reduced this increase. found to be significantly suppressed.
A Lactobacillus bacterium was isolated and identified as Lam-1, which is highly sensitive to penicillin. The researchers found that treatment of mice with penicillin significantly suppressed Lam-1 growth, attenuated intestinal lipid absorption, decreased fatty acid uptake in WAT, and decreased body fat accumulation after dieting. discovered to do
On the other hand, tests in germ-free (GF), gnotobiotic (GB), and specific pathogen-free (SPF) mice showed that supplementation with Lam-1 or its metabolites significantly enhanced intestinal lipid absorption and in WAT. was shown to increase the fatty acid uptake ofled to fat mass Increase and obesity.
This study shows that refeeding after DR contributes to the development of obesity and provides an important animal model for obesity research. Also, targeting lactobacilli using a high-protein diet or antibiotics to inhibit intestinal lipid absorption may effective strategy To prevent obesity after dieting.
Wuling Zhong et al. A high-protein diet prevents post-diet fat gain by counteracting Lactobacillus-enhanced lipid absorption. natural metabolism(2022). DOI: 10.1038/s42255-022-00687-6
Amir Zarrinpar, High protein diet prevents weight regain, natural metabolism(2022). DOI: 10.1038/s42255-022-00699-2
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Chinese Academy of Sciences
Quote: Researchers Reveal Why Fat Gains After Dieting and How Protein Can Help (Dec 5, 2022)
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